Journal: Oncotarget

Article Title: Evaluation of cancer-derived myocardial impairments using a mouse model

doi: 10.18632/oncotarget.27759

Figure Lengend Snippet: ( A ) Immunostaining of 8-OHdG (oxidative stress). Scale bar, 50 μm. ( B ) Western blot analysis of LETM1 (mitochondria). ( C ) Western blot analysis of LC3. LC3-II is a marker for autophagy. ( D ) Semi-quantification of LETM1 and LC3-II. ( E ) SDS-soluble myosin light chain-2 (MYL1) measured using ELISA. The signals were standardized with the β-actin signal. Error bar, standard error of data collected from 3 mice. Significant differences were calculated using Student t -test. 8-OHdG, 8-hydroxy-2′-deoxyguanosine; LETM1, leucine zipper and EF-hand-containing transmembrane protein 1; LC3, microtubule-associated protein light chain-3; Control, no tumor control; CT26, mice inoculated with CT26 cells intraperitoneally; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Anti-8-hydroxy-2′-deoxyguanosine (8-OHdG) antibody (Japan Institute for the Control of Aging, NIKKEN SEIL Co., Ltd., Shizuoka, Japan) was used to confirm the accumulation of oxidative stress in the nuclei.

Techniques: Immunostaining, Western Blot, Marker, Enzyme-linked Immunosorbent Assay

Journal: Human Molecular Genetics

Article Title: Mitochondria-targeted catalase reduces abnormal APP processing, amyloid ? production and BACE1 in a mouse model of Alzheimer's disease: implications for neuroprotection and lifespan extension

doi: 10.1093/hmg/dds128

Figure Lengend Snippet: 8-OHdG quantification, using high pressure liquid chromatography (HPLC) analysis of the cortical and hippocampal tissues from the brains of transgenic and WT mice. (A) Changes in the levels of 8-OHdG in all four genotype groups (*P < 0.05; **P < 0.005). (B) Differences across the ages (*P < 0.05). (C) Levels of 8-OHdG in the AβPP and MCAT/AβPP mice at the three ages (*P < 0.05; **P < 0.01).

Article Snippet: Details of the antibodies are given in Table . table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody Secondary antibody Species and dilution Purchased from Dilution, Alexa dye Purchased from Anti-Aβ (6E10) Mouse monoclonal 1:200 Covance, San Diego, CA, USA Goat anti-mouse biotin 1:400, ABC, TSA-Alexa594 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA Anti-Aβ42 (AB05) Mouse monoclonal (ready to use from the kit) Wako Pure Chemicals, Japan Goat anti-mouse biotin, ABC solution, TSA-Alexa594 Wako Pure Chemicals, Japan Anti-Aβ40 (BA27) Mouse monoclonal (ready to use from the kit) Wako Pure Chemicals, Japan Goat anti-mouse biotin, ABC solution, TSA-Alexa594 Wako Pure Chemicals, Japan Catalase Rabbit polyclonal 1:200 Abcam, Cambridge, MA, USA Goat anti-rabbit biotin 1:400, ABC, TSA-Alexa 488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA BACE1 Rabbit polyclonal 1:150 Calbiochem, San Diego, CA, USA Goat anti-rabbit biotin 1:400, ABC,TSA-Alexa488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA 8-OHdG Goat polyclonal, 1:200 Neuromics, Edina, MN, USA Horse anti-goat biotin, 1:400, ABC,TSA-Alexa488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence using brain sections from transgenic mice and control WT mice We quantified Aβ40- and Aβ42-specific deposits.

Techniques: High Performance Liquid Chromatography, Transgenic Assay